Fig. 1

c-Jun induced neuroblastoma cell differentiation. (A). c-Jun expression in different neuroblastoma cell lines and glioblastoma cell lines deposited at The Human Protein Atlas. (B). SH-SY5Y cells were induced for differentiation by RA and expression of c-Jun protein during differentiation was detected by Western blot (n = 3). (C). Ch-IP assay by using c-Jun antibody to detect the binding of c-Jun onto the promoters of PDGFRα, RARα, RARβ and RARγ. (D). Immunoblots showing the expression of differentiation markers in control cells and c-Jun OE cells after RA-induced differentiation. (E-G). Relative protein level of TUBB3, GAP43 and c-Jun were analyzed by unpaired t-test (mean ± SEM of triplicate experiments). *p < 0.05, **p < 0.01. (H). Neural differentiation of negative control (NC, cells transfected with Lentiviral vector) and c-Jun OE SH-SY5Y cells. Cells were stained with TUBB3 and DAPI, and images were acquired using a fluorescence microscope (Nikon). (I). Number of cells with neurite outgrowth (> 20 μm) was counted using Image J software. Pictures were taken from 3–5 areas of each well with at least three independent experiments. The mean of each well was used for statistical analysis with unpaired t-test. Means with SEM were shown. *p < 0.05, **p < 0.01, ***p < 0.001